Gene Control during Transcription Elongation

Gene Control during Transcription Elongation

1Departamento de Genética, Facultad de Bioloǵıa, Universidad de Sevilla, Seville 41012, Spain 2Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130-3932, USA 3Laboratoire de Genetique Moleculaire, Academie Universitaire Louvain, 5000 Namur, Belgium 4Departamento de Bioloǵıa Molecular, Instituto de Parasitoloǵıa y Biomedicina “...

Dynamic enhancer-gene body contacts during transcription elongation.

Enhancers govern transcription through multiple mechanisms, including the regulation of elongation by RNA polymerase II (RNAPII). We characterized the dynamics of looped enhancer contacts during synchronous transcription elongation. We found that many distal enhancers form stable contacts with their target promoters during the entire interval of elongation. Notably, we detected additional dynam...

Transcription elongation

Control of transcription elongation by GreA determines rate of gene expression in Streptococcus pneumoniae

Transcription by RNA polymerase may be interrupted by pauses caused by backtracking or misincorporation that can be resolved by the conserved bacterial Gre-factors. However, the consequences of such pausing in the living cell remain obscure. Here, we developed molecular biology and transcriptome sequencing tools in the human pathogen Streptococcus pneumoniae and provide evidence that transcript...

The Prefoldin Complex Regulates Chromatin Dynamics during Transcription Elongation

Transcriptional elongation requires the concerted action of several factors that allow RNA polymerase II to advance through chromatin in a highly processive manner. In order to identify novel elongation factors, we performed systematic yeast genetic screening based on the GLAM (Gene Length-dependent Accumulation of mRNA) assay, which is used to detect defects in the expression of long transcrip...